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koerber  (Addgene inc)


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    Addgene inc koerber
    Koerber, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmscvpuro-flag/pMSCVpuro-Flag-cMyc+T58A+(Plasmid+%2320076)/pm40966085-407-9-29
    Average 92 stars, based on 10 article reviews
    koerber - by Bioz Stars, 2026-09
    92/100 stars

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    Generated:

    Article Title: Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex
    Article Snippet: We obtained and engineered pMSCVpuro-FLAG and pMSCVhygro-FLAG vectors from Addgene (#53178, #75083). pLKO.1 puro-shBECN1 and pLKO.1 hygro-shRIPK3 were generated using oligo annealing and cloning into an empty vector using following primers: shBECN1 #5F 5′-CCGGGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATCTTTTTG-3’, R 5′-AATTCAAAAAGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATC-3′, shBECN1 #7F 5′-CCGGCTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAGTTTTTG-3′, R 5′-AATTCAAAAACTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAG-3′, shRIPK3 F 5′-CCGGAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTTTTTTTG-3′, R 5′-AATTCAAAAAAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTT-3′. shMLKL (TRCN0000196317) was purchased from Sigma-Aldrich.

    Clone Assay:

    Article Title: Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex
    Article Snippet: We obtained and engineered pMSCVpuro-FLAG and pMSCVhygro-FLAG vectors from Addgene (#53178, #75083). pLKO.1 puro-shBECN1 and pLKO.1 hygro-shRIPK3 were generated using oligo annealing and cloning into an empty vector using following primers: shBECN1 #5F 5′-CCGGGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATCTTTTTG-3’, R 5′-AATTCAAAAAGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATC-3′, shBECN1 #7F 5′-CCGGCTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAGTTTTTG-3′, R 5′-AATTCAAAAACTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAG-3′, shRIPK3 F 5′-CCGGAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTTTTTTTG-3′, R 5′-AATTCAAAAAAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTT-3′. shMLKL (TRCN0000196317) was purchased from Sigma-Aldrich.

    Plasmid Preparation:

    Article Title: Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex
    Article Snippet: We obtained and engineered pMSCVpuro-FLAG and pMSCVhygro-FLAG vectors from Addgene (#53178, #75083). pLKO.1 puro-shBECN1 and pLKO.1 hygro-shRIPK3 were generated using oligo annealing and cloning into an empty vector using following primers: shBECN1 #5F 5′-CCGGGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATCTTTTTG-3’, R 5′-AATTCAAAAAGATACCGACTTGTTCCTTACGCTCGAGCGTAAGGAACAAGTCGGTATC-3′, shBECN1 #7F 5′-CCGGCTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAGTTTTTG-3′, R 5′-AATTCAAAAACTAAGGAGCTGCCGTTATACTCTCGAGAGTATAACGGCAGCTCCTTAG-3′, shRIPK3 F 5′-CCGGAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTTTTTTTG-3′, R 5′-AATTCAAAAAAACCAGCACTCTCGTAATGATCTCGAGATCATTACGAGAGTGCTGGTT-3′. shMLKL (TRCN0000196317) was purchased from Sigma-Aldrich.



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    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: KRAS Suppression-Induced Degradation of MYC is Antagonized by a MEK5-ERK5 Compensatory Mechanism

    doi: 10.1016/j.ccell.2018.10.001

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: The pMSCV puro retrovirus vector and pMSCV puro encoding FLAG epitope-tagged MYC (T58A) were provided by Juan Belmonte (Addgene plasmid 20076) ( Aasen et al., 2008 ). pMSCV puro vectors encoding FLAG epitope-tagged MYC S62A and S58/62A were generated as we described previously ( Hayes et al., 2016 ).

    Techniques: Recombinant, In Vivo, Viability Assay, Software